MultiBarcodeTools
Choose multiple barcodes customized for your research
Due to the limitation of short amplicon length, it is usually difficult to unambiguously distinguish all the species in the sample through a single amplicon. In this situation, the multi-barcode strategy, that is, a combination of barcodes, is necessary.
MultiBarcodeTools help you find an ideal combination that can:
- use the minimum barcodes
- distinguish the most amount of species
a web platform that can automatically selects the optimal combination of fish eDNA multi-metabarcoding primers based on a given list of potential target species and research requirements.
a web platform that display combination of barcodes based on observed fishes in different basins and marine regions in Japan.
a pipeline that helps to select a combination of barcodes based on amplicon sequences provided by users.